opentrons-ot2-protocols

Automate liquid handling protocols for Opentrons OT-2 and Flex robots using Python.

6|2|Updated Jun 11, 2026

Search Tags:#robotics #protocols #opentrons #liquid handling #pcr

Ready to run

npx skills add https://github.com/pradyumnasagar/open-research-skills --skill opentrons-ot2-protocols

💬 Prompt for ChatGPT / Claude Chat▼

Please help me install this Agent Skill.
Skill: opentrons-ot2-protocols
Source: https://github.com/pradyumnasagar/open-research-skills/tree/main/skills/lab-automation/opentrons-ot2-protocols
Command: npx skills add https://github.com/pradyumnasagar/open-research-skills --skill opentrons-ot2-protocols

SYSTEM DOCUMENTATION & REQUIREMENTS

💡 This Skill requires opentrons, and includes scripts (resource) components.

What problem does it solve?

This Skill automates complex liquid handling tasks on Opentrons OT-2 and Flex robots, streamlining PCR, ELISA, plate stamping, and magnetic bead cleanup protocols.

Core Features & Use Cases

Protocol Automation: Write and execute liquid handling protocols in Python.
Hardware Integration: Control thermocyclers, heater-shakers, magnetic modules, and more.
Simulation: Test protocols locally with opentrons_simulate before robot runs.
Use Case: For PCR, the skill can set up reactions, distribute master mix, and run thermal profiles. For ELISA, it can perform plate layouts, blocking, and detection.

Quick Start

Use the opentrons-ot2-protocols skill to run a PCR setup protocol with 'pcr_protocol.py'.

Frequently Asked Questions about opentrons-ot2-protocols

High-intent search queries and answers about installing and using this skill.

FAQPage Schema

How do I automate PCR setup and liquid handling protocols for an Opentrons robot?▼

You can automate PCR setup by writing Python liquid handling protocols using the Opentrons API v2. This skill generates scripts to distribute master mix and control thermocyclers on OT-2 and Flex robots.

Do I need the opentrons library to simulate liquid handling protocols locally?▼

Yes, you need the opentrons library installed to use opentrons_simulate for testing liquid handling protocols. This allows you to validate Python scripts locally before executing them on the physical robot.

Can I control magnetic modules and heater-shakers for ELISA protocols with Opentrons?▼

Yes, this skill supports hardware integration for ELISA workflows by controlling magnetic modules, heater-shakers, and thermocyclers. You can automate plate layouts, blocking, and detection steps in Python.

What is the best way to write Python scripts for magnetic bead cleanup on OT-2 robots?▼

The best way to write magnetic bead cleanup scripts is using Opentrons API v2 in Python. This skill provides the structural logic to automate bead separation and washing workflows on OT-2 and Flex robots.

Does this skill support plate stamping protocols for Opentrons Flex robots?▼

Yes, this skill automates plate stamping protocols alongside PCR and ELISA workflows for Opentrons Flex and OT-2 robots. It uses Python scripts to manage the liquid handling transfers required for plate stamping.