rrna-prediction-patterns

Detect and predict rRNA sequences using HMM models and BLAST searches.

Updated Nov 20, 2025
One-click install
npx skills add https://github.com/roeimed0/rrna-phylo --skill rrna-prediction-patterns
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: rrna-prediction-patterns
Source: https://github.com/roeimed0/rrna-phylo/tree/main/.claude/skills/rrna-prediction-patterns
Command: npx skills add https://github.com/roeimed0/rrna-phylo --skill rrna-prediction-patterns

SYSTEM DOCUMENTATION & REQUIREMENTS

What problem does it solve?

This Skill provides comprehensive guidelines for implementing ribosomal RNA detection and prediction across prokaryotic and eukaryotic organisms. It covers sequence patterns, secondary structure considerations, HMM models, BLAST-based detection, length validation, and quality scoring to ensure accurate identification and robust validation.

Core Features & Use Cases

  • Multi-method detection: HMM, BLAST, and conserved-pattern searches to identify rRNA types (16S, 18S, 23S, 28S, 5S, 5.8S).
  • Length validation & conservation checks: Validate sequence length against expected ranges and verify conserved regions for completeness.
  • Quality scoring & error handling: Provide confidence measures and gracefully handle partial or ambiguous predictions.
  • Use Case: Integrate into bioinformatics pipelines to annotate rRNA genes in bacterial and eukaryotic genomes, enable quality control, and support phylogenetic analyses.

Quick Start

Review the SKILL.md to incorporate HMM-based, BLAST-based, and pattern-driven rRNA detection into your pipeline.

Frequently Asked Questions about rrna-prediction-patterns

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I detect 16S and 23S rRNA sequences in a prokaryotic genome?

To detect rRNA sequences, apply multi-method workflows combining HMM-based models, BLAST searches, and conserved-pattern detection to classify 16S, 23S, and 5S rRNAs across prokaryotic genomes.

What is the best way to validate predicted rRNA sequences for completeness?

rRNA validation relies on sequence-length checks against expected ranges and conservation-region pattern verification to ensure sequence completeness and produce reliable, well-annotated predictions.

How do I identify eukaryotic 18S and 28S rRNA using bioinformatics pipelines?

Eukaryotic rRNA identification uses HMM-based models and BLAST searches to detect 18S, 28S, and 5.8S rRNA sequences, enforcing multi-method validation and quality scoring for accurate phylogenetic annotation.

Can I use HMM and BLAST together for ribosomal RNA detection?

Yes, combining HMM and BLAST searches with conserved-pattern detection provides multi-method validation, ensuring robust rRNA prediction and high-confidence classification for both prokaryotic and eukaryotic organisms.

How do you handle partial or ambiguous rRNA predictions during genome annotation?

Partial or ambiguous rRNA predictions are managed through quality scoring mechanisms that provide confidence measures and enforce multi-method validation, allowing graceful error handling during sequence annotation.

Why does my rRNA prediction workflow return sequences with incorrect lengths?

Incorrect rRNA sequence lengths occur when validation steps are skipped; applying length validation against expected ranges and verifying conserved regions ensures completeness and prevents inaccurate predictions.