scientific-rrna-taxonomy

Classify rRNA amplicon sequences against SILVA/Greengenes2 references and MGnify taxonomy.

3|1|Updated Feb 11, 2026
One-click install
npx skills add https://github.com/nahisaho/satori --skill scientific-rrna-taxonomy
Or copy as Structured Prompt for Agent
Please help me install this Agent Skill.
Skill: scientific-rrna-taxonomy
Source: https://github.com/nahisaho/satori/tree/main/src/.github/skills/scientific-rrna-taxonomy
Command: npx skills add https://github.com/nahisaho/satori --skill scientific-rrna-taxonomy

SYSTEM DOCUMENTATION & REQUIREMENTS

What problem does it solve?

Efficiently annotate and classify ribosomal RNA sequences (16S/18S/ITS) by leveraging established reference databases and taxonomic pipelines to deliver consistent taxonomic labels.

Core Features & Use Cases

  • Reference-driven taxonomy assignment using SILVA/Greengenes2 and MGnify integration.
  • Support for 16S/18S/ITS amplicons and comparative taxonomic profiling across experiments.
  • Easy integration with QIIME2 pipelines and custom classifier training for reproducible analyses.

Quick Start

Classify your rRNA amplicon FASTA file against SILVA/Greengenes2 references and MGnify to obtain taxonomic assignments.

Frequently Asked Questions about scientific-rrna-taxonomy

High-intent search queries and answers about installing and using this skill.

FAQPage Schema
How do I classify rRNA amplicon sequences against the SILVA reference database?

You can classify your rRNA amplicon sequences by inputting a standard FASTA file to match against SILVA or Greengenes2 references and MGnify taxonomy, generating consistent taxonomic labels for your sequences.

Can I use this taxonomy assignment workflow with QIIME2 pipelines?

Yes, the rRNA taxonomy assignment supports seamless integration with QIIME2 pipelines, allowing you to incorporate reference-driven classification and custom classifier training directly into your reproducible analysis workflows.

Does this rRNA taxonomy tool support 18S and ITS amplicons, or just 16S?

The rRNA taxonomy tool supports 16S, 18S, and ITS amplicons, enabling comprehensive reference-driven taxonomy assignment and comparative taxonomic profiling across diverse microbiome and environmental datasets.

What is the best way to compare taxonomic profiles across different experiments using MGnify?

Comparing taxonomic profiles across experiments is achieved by leveraging MGnify integration, which aligns your rRNA amplicon classification results against standardized reference databases to deliver consistent taxonomic labels.

Do I need to train a custom classifier for taxonomy assignment with Greengenes2?

While reference databases like Greengenes2 are required for classification, the workflow supports custom classifier training, allowing you to tailor the taxonomy assignment process for highly reproducible analyses.

What reference databases are required for assigning taxonomy to environmental rRNA datasets?

Assigning taxonomy to environmental rRNA datasets requires established reference databases such as SILVA and Greengenes2, along with MGnify integration, to accurately annotate and classify your ribosomal RNA sequences.